| First Author | Lüönd F | Year | 2019 |
| Journal | J Mammary Gland Biol Neoplasia | Volume | 24 |
| Issue | 1 | Pages | 39-45 |
| PubMed ID | 30209717 | Mgi Jnum | J:335073 |
| Mgi Id | MGI:7465242 | Doi | 10.1007/s10911-018-9412-4 |
| Citation | Luond F, et al. (2019) A Transgenic MMTV-Flippase Mouse Line for Molecular Engineering in Mammary Gland and Breast Cancer Mouse Models. J Mammary Gland Biol Neoplasia 24(1):39-45 |
| abstractText | Genetically engineered mouse models have become an indispensable tool for breast cancer research. Combination of multiple site-specific recombination systems such as Cre/loxP and Flippase (Flp)/Frt allows for engineering of sophisticated, multi-layered conditional mouse models. Here, we report the generation and characterization of a novel transgenic mouse line expressing a mouse codon-optimized Flp under the control of the mouse mammary tumor virus (MMTV) promoter. These mice show robust Flp-mediated recombination in luminal mammary gland and breast cancer cells but no Flp activity in non-mammary tissues, with the exception of limited activity in salivary glands. These mice provide a unique tool for studying mammary gland biology and carcinogenesis in mice. |
