| First Author | Hernández-Fernaud JR | Year | 2010 |
| Journal | FEBS J | Volume | 277 |
| Issue | 22 | Pages | 4766-74 |
| PubMed ID | 20977670 | Mgi Jnum | J:177901 |
| Mgi Id | MGI:5296452 | Doi | 10.1111/j.1742-4658.2010.07882.x |
| Citation | Hernandez-Fernaud JR, et al. (2010) Differential expression of liver and kidney proteins in a mouse model for primary hyperoxaluria type I. FEBS J 277(22):4766-74 |
| abstractText | Mutations in the alanine-glyoxylate aminotransferase gene (AGXT) are responsible for primary hyperoxaluria type I, a rare disease characterized by excessive hepatic oxalate production that leads to renal failure. A deeper understanding of the changes in the metabolic pathways secondary to the lack of AGXT expression is needed in order to explore substrate depletion as a therapeutic strategy to limit oxalate production in primary hyperoxaluria type I. We have developed an Agxt knockout (AgxtKO) mouse that reproduces some key features of primary hyperoxaluria type I. To improve our understanding of the metabolic adjustments subsequent to AGXT deficiency, we performed a proteomic analysis of the changes in expression levels of various subcellular fractions of liver and kidney metabolism linked to the lack of AGXT. In this article, we report specific changes in the liver and kidney proteome of AgxtKO mice that point to significant variations in gluconeogenesis, glycolysis and fatty acid pathways. |
