| Primary Identifier | MGI:3528074 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Drd5 |
| Transmission | Germline | Strain of Origin | 129S4/SvJae |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A neo was inserted in reverse orientation to disrupt the reading frame within the coding region. A stop codon was engineered into the proximal neo linker to prematurely truncate the transcript subsequent to Gly-190 in the second extracellular loop. RT-PCR verified presence of the recombinant transcript in mutant brains. Immunohistochemistry using peptides from the region downstream of the stop codon showed that mutants did not produce the corresponding protein. |
