| First Author | Lei WL | Year | 2023 |
| Journal | Int J Biol Sci | Volume | 19 |
| Issue | 15 | Pages | 4883-4897 |
| PubMed ID | 37781512 | Mgi Jnum | J:357753 |
| Mgi Id | MGI:7537933 | Doi | 10.7150/ijbs.83474 |
| Citation | Lei WL, et al. (2023) SRSF1-mediated alternative splicing is required for spermatogenesis. Int J Biol Sci 19(15):4883-4897 |
| abstractText | Alternative splicing (AS) plays significant roles in a multitude of fundamental biological activities. AS is prevalent in the testis, but the regulations of AS in spermatogenesis is only little explored. Here, we report that Serine/arginine-rich splicing factor 1 (SRSF1) plays critical roles in alternative splicing and male reproduction. Male germ cell-specific deletion of Srsf1 led to complete infertility by affecting spermatogenesis. Mechanistically, by combining RNA-seq data with LACE-seq data, we showed that SRSF1 affected the AS of Stra8 in a direct manner and Dazl, Dmc1, Mre11a, Syce2 and Rif1 in an indirect manner. Our findings demonstrate that SRSF1 has crucial functions in spermatogenesis and male fertility by regulating alternative splicing. |
