| First Author | Fos C | Year | 2014 |
| Journal | Sci Signal | Volume | 7 |
| Issue | 345 | Pages | ra93 |
| PubMed ID | 25270259 | Mgi Jnum | J:259968 |
| Mgi Id | MGI:6141066 | Doi | 10.1126/scisignal.2005565 |
| Citation | Fos C, et al. (2014) Association of the EF-hand and PH domains of the guanine nucleotide exchange factor SLAT with IP(3) receptor 1 promotes Ca(2)(+) signaling in T cells. Sci Signal 7(345):ra93 |
| abstractText | The guanine nucleotide exchange factor SLAT (SWAP-70-like adaptor of T cells) regulates T cell activation and differentiation by enabling Ca(2+) release from intracellular stores in response to stimulation of the T cell receptor (TCR). We found a TCR-induced association between SLAT and inositol 1,4,5-trisphosphate (IP3) receptor type 1 (IP3R1). The N-terminal region of SLAT, which contains two EF-hand motifs that we determined bound Ca(2+), and the SLAT pleckstrin homology (PH) domain independently bound to IP3R1 by associating with a conserved motif within the IP3R1 ligand-binding domain. Disruption of the SLAT-IP3R1 interaction with cell-permeable, IP3R1-based fusion peptides inhibited TCR-stimulated Ca(2+) signaling, activation of the transcription factor NFAT (nuclear factor of activated T cells), and production of cytokines, suggesting that this interaction is required for optimal T cell activation. The finding that SLAT is an IP3R1-interacting protein required for T cell activation suggests that this interaction could be a potential target for a selective immunosuppressive drug. |
