| Primary Identifier | MGI:3531483 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Dmd |
| Transmission | Germline | Strain of Origin | (C57BL/6NCrlj x CBA/JNCrlj)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | 3' targeting vector containing a loxP site was inserted downstream of exon 79. Subsequently 5' targeting vector containing second loxP site was inserted to replace brain-type promoter and its specific exon 1. In resulting mutant animals, the entire region of the Dmd gene was flanked by two loxP sites, and full-length expression in the brain was specifically inactivated. RT-PCR and Western blot analyses showed full length dystrophin expression in the cerebrum was specifically inactivated, with no effect on expressions of other isoforms. |
