Primary Identifier | MGI:5788158 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change, Reporter | Gene | Nfkb2 |
Transmission | Germline | Strain of Origin | (C57BL/6J x 129S6/SvEvTac)F1 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Sequence encoding a promoterless eGFP minigene, a loxP site and the PGKneo cassette is inserted upstream of exon 1 of the targeted gene, and another loxP site followed by the PGK promoter is inserted downstream of exon 2. The translation start site is in exon 2. The entire targeted region is flanked by FRT sites. Recombination using Cre recombinase will remove the exons while allowing EGFP expression or FLP-mediated recombination will remove both the exons and the EGFP. |