| First Author | Oleskevich S | Year | 2005 |
| Journal | Neuroreport | Volume | 16 |
| Issue | 9 | Pages | 921-5 |
| PubMed ID | 15931062 | Mgi Jnum | J:103570 |
| Mgi Id | MGI:3610424 | Doi | 10.1097/00001756-200506210-00009 |
| Citation | Oleskevich S, et al. (2005) Enhanced serotonin response in the hippocampus of Galphaz protein knock-out mice. Neuroreport 16(9):921-5 |
| abstractText | The serotonin-1A [5-hydroxytryptamine 1A (5HT1A)] receptor is important for emotional and homeostatic processes in the central nervous system. In the hippocampus, the 5HT1A receptor couples to inhibitory Gi/o proteins to decrease pyramidal cell excitability. Here we investigate the 5HT1A receptor in a mouse deficient in the alpha-subunit of Gz protein (Galphaz knock-out). Behavioural tests showed heightened anxiety and depression-like behaviour in the Galphaz knock-out mice. Whole-cell recording in CA1 pyramidal neurons showed a significantly greater 5HT1A receptor-mediated potassium current in Galphaz knock-out mice. The effect was independent of 5HT4 receptors as the slow after-hyperpolarization was unaffected and a slow depolarization was absent in the Galphaz knock-out mice. Other receptors linked to Gi/o proteins [gamma-aminobutyric acid type B receptor (GABAB), adenosine A1 and muscarinic acetylcholine receptors] were not affected in Galphaz knock-out mice. These results suggest that the 5HT1A receptor may be linked to Galphaz protein, as reported previously in cell culture but shown here in an intact neural network. |
