Primary Identifier | MGI:3576227 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Park7 |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Exons 3-5 were replaced with a neomycin resistance gene. Exon 1 was modified to contain a premature stop codon, resulting in a transcript containing only the first 8 amino acids. Absence of transcript was confirmed by Northern blot of RNA from mutant MEFs. Absence of protein was shown by Western blot of mutant cortical neurons. |