| Primary Identifier | MGI:3582685 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Gata1 |
| Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An AvrII-NheI genomic fragment spanning exon 2 and part of exon 3 was amplified. Then a 5' loxP site was introduced to the AvrII site and a neo cassette was inserted into the PstI site in the intron between exons 2 and 3. The neo cassette was later removed by transfecting a GFP-Cre plasmid and then carrying out fluorescence-activated cell sorting for GFP+ cells, leaving exon 2 flanked by loxP sites. |
