| Primary Identifier | MGI:3583916 | Allele Type | Targeted |
| Gene | Tnf | Transmission | Germline |
| Strain of Origin | Not Specified | Is Recombinase | false |
| Is Wild Type | false |
| description | ES cell line = W9. |
| molecularNote | The 3' UTR of Gapdh, bearing two missense mutations in the trimer interface region, was substituted in place of the endogenous 3' UTR via homologous recombination. The loxP flanked neomycin resistance cassette was excised out after selection by transient transfection of the surviving ES cells with pCMB-cre. |
