| First Author | Chen YT | Year | 2000 |
| Journal | Genesis | Volume | 28 |
| Issue | 1 | Pages | 31-5 |
| PubMed ID | 11020714 | Mgi Jnum | J:99558 |
| Mgi Id | MGI:3582942 | Doi | 10.1002/1526-968x(200009)28:1<31::aid-gene40>3.0.co;2-k |
| Citation | Chen YT, et al. (2000) A new positive/negative selectable marker, puDeltatk, for use in embryonic stem cells. Genesis 28(1):31-5 |
| abstractText | A novel positive/negative selection cassette, puDeltatk, was generated. pu(Delta)tk is a bifunctional fusion protein between puromycin N-acetyltransferase (Puro) and a truncated version of herpes simplex virus type 1 thymidine kinase (DeltaTk). Murine embryonic stem (ES) cells transfected with pu(Delta)tk become resistant to puromycin and sensitive to 1-(-2-deoxy-2-fluoro-1-beta-D-arabino-furanosyl)-5-iodouracil (FIAU). Unlike other HSV1 tk transgenes, puDeltatk is readily transmitted through the male germ line. Thus pu(Delta)tk is a convenient positive/negative selectable marker that can be widely used in many ES cell applications. |
