| First Author | Inoue K | Year | 2009 |
| Journal | J Reprod Dev | Volume | 55 |
| Issue | 5 | Pages | 566-9 |
| PubMed ID | 19602850 | Mgi Jnum | J:346838 |
| Mgi Id | MGI:6511831 | Doi | 10.1262/jrd.09-099e |
| Citation | Inoue K, et al. (2009) Sex-reversed somatic cell cloning in the mouse. J Reprod Dev 55(5):566-9 |
| abstractText | Somatic cell nuclear transfer has many potential applications in the fields of basic and applied sciences. However, it has a disadvantage that can never be overcome technically-the inflexibility of the sex of the offspring. Here, we report an accidental birth of a female mouse following nuclear transfer using an immature Sertoli cell. We produced a batch of 27 clones in a nuclear transfer experiment using Sertoli cells collected from neonatal male mice. Among them, one pup was female. This "male-derived female" clone grew into a normal adult and produced offspring by natural mating with a littermate. Chromosomal analysis revealed that the female clone had a 39,X karyotype, indicating that the Y chromosome had been deleted in the donor cell or at some early step during nuclear transfer. This finding suggests the possibility of resuming sexual reproduction after a single male is cloned, which should be especially useful for reviving extinct or endangered species. |
