| First Author | Forster A | Year | 2005 |
| Journal | Nat Methods | Volume | 2 |
| Issue | 1 | Pages | 27-30 |
| PubMed ID | 15782166 | Mgi Jnum | J:98679 |
| Mgi Id | MGI:3579619 | Doi | 10.1038/nmeth727 |
| Citation | Forster A, et al. (2005) The invertor knock-in conditional chromosomal translocation mimic. Nat Methods 2(1):27-30 |
| abstractText | Knock-in models of tumor-specific chromosomal translocations can generate lethal mutations. To circumvent this, a new conditional gene fusion model has been developed (invertor mice) and exemplified with the Ews-ERG fusion oncogene. An ERG segment, flanked by loxP sites, was knocked in to an intron of the Ews gene but in an inverted transcription orientation and lineage-specific Ews-ERG fusion created by Cre-mediated inversion. This invertor method is a completely conditional approach, applicable to any gene fusion, to emulate effects of translocations found in human cancers. |
