| Primary Identifier | MGI:3584174 | Allele Type | Targeted |
| Attribute String | Reporter | Gene | Tsc1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A beta-galactosidase cassette with a floxed neomycin resistance gene was inserted to replace the 3' half of exon 3 and all of exons 7 and 8. RT-PCR demonstrated that this replacement truncated the coding sequence in exon 6 by introducing TAG stop codons in all three reading frames and created a bicistronic transcript where beta-galactosidase was under control of the endogenous regulatory sequences by an IRES. |
