| First Author | Hueber AO | Year | 1997 |
| Journal | Curr Biol | Volume | 7 |
| Issue | 9 | Pages | 705-8 |
| PubMed ID | 9285719 | Mgi Jnum | J:42939 |
| Mgi Id | MGI:1096761 | Doi | 10.1016/s0960-9822(06)00300-9 |
| Citation | Hueber AO, et al. (1997) Thymocytes in Thy-1-/- mice show augmented TCR signaling and impaired differentiation. Curr Biol 7(9):705-8 |
| abstractText | Thy-1, a single variable-like immunoglobulin superfamily domain anchored in the plasma membrane by a glycosyl phosphaditylinositol tail [1], is a major surface glycoprotein in adult mammalian neurons and rodent thymocytes [2]; the function of Thy-1 has remained enigmatic since its discovery [3]. Studies in vitro have implicated Thy-1 in homotypic and heterotypic cell-cell interactions [2,4]. Ligation of Thy-1 initiates transmembrane signaling pathways that lead to diverse physiological outcomes in different cells [2,5-7]. In rodents, Thy-1 is highly expressed on the surface of CD4+CD8+ double-positive immature thymocytes and downregulated in mature T cells. Here, we report that thymocytes from Thy-1-/- mice [8] had altered cell-cell contacts, and hyperresponsiveness to T-cell receptor (TCR) triggering as demonstrated by the heightened activation of p56lck, phosphorylation of TCR subunits, Ca2+ fluxes and cell proliferation. Thy-1-/- thymocytes exhibited impaired maturation from the double positive to single positive stage of thymocyte development, possibly due to inappropriate negative selection, and were prone to T lymphomas in aged mice. These observations indicate that Thy-1 negatively regulates TCR-mediated signaling and controls activation thresholds during thymocyte differentiation. |
