| First Author | Spoljaric I | Year | 2019 |
| Journal | Cell Rep | Volume | 26 |
| Issue | 5 | Pages | 1073-1081.e3 |
| PubMed ID | 30699338 | Mgi Jnum | J:288517 |
| Mgi Id | MGI:6432195 | Doi | 10.1016/j.celrep.2019.01.011 |
| Citation | Spoljaric I, et al. (2019) KCC2-Mediated Cl(-) Extrusion Modulates Spontaneous Hippocampal Network Events in Perinatal Rats and Mice. Cell Rep 26(5):1073-1081.e3 |
| abstractText | It is generally thought that hippocampal neurons of perinatal rats and mice lack transport-functional K-Cl cotransporter KCC2, and that Cl(-) regulation is dominated by Cl(-) uptake via the Na-K-2Cl cotransporter NKCC1. Here, we demonstrate a robust enhancement of spontaneous hippocampal network events (giant depolarizing potentials [GDPs]) by the KCC2 inhibitor VU0463271 in neonatal rats and late-gestation, wild-type mouse embryos, but not in their KCC2-null littermates. VU0463271 increased the depolarizing GABAergic synaptic drive onto neonatal CA3 pyramidal neurons, increasing their spiking probability and synchrony during the rising phase of a GDP. Our data indicate that Cl(-) extrusion by KCC2 is involved in modulation of GDPs already at their developmental onset during the perinatal period in mice and rats. |
