| Primary Identifier | MGI:3590151 | Allele Type | Targeted |
| Attribute String | Modified regulatory region, Null/knockout | Gene | Rr149356 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The 560-bp HpaIâNcoI restriction fragment containing Tcrb enhancer Ebeta was replaced with a loxP site flanked neomycin resistance gene cassette. The neo cassette was removed through subsequent cre-mediated recombination. Mutants were shown to lack Tcrb rearrangement by DNA-PCR and RNA-PCR analyses although germ-line Vbeta transcripts were detectable at a low level. |
