| Primary Identifier | MGI:3590580 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Rho |
| Transmission | Germline | Strain of Origin | 129S4/SvJae |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting construct was designed to insert Cys322Thr and Cys323Ser modifications in the locus to eliminate both palmitoylation sites. A tACE-Cre-neo cassette was included in the construct and self-excised upon passage through the male germ line. Expression of palmitoylation-deficient rhodopsin transcript was demonstrated by RT-PCR, and the absence of palmitates on rhodopsin protein of mutant mice was confirmed by mass spectrometry. |
