| First Author | Hara T | Year | 2012 |
| Journal | Anticancer Res | Volume | 32 |
| Issue | 8 | Pages | 3097-101 |
| PubMed ID | 22843879 | Mgi Jnum | J:320932 |
| Mgi Id | MGI:6882076 | Citation | Hara T, et al. (2012) PKCalpha suppresses 7,12-dimethylbenz[a]anthracene-induced skin tumor formation. Anticancer Res 32(8):3097-101 |
| abstractText | BACKGROUND: Protein kinase C (PKC)alpha is distributed in almost all tissues and participates in various signaling pathways. However, the role of PKCalpha in carcinogenesis remains unclear. In this study, we performed complete skin carcinogenesis in PKCalpha knockout mice by repeated administration of 7,12-dimethylbenz[a]anthracene (DMBA). MATERIALS AND METHODS: Complete skin carcinogenesis was performed by repeated DMBA treatment using PKCalpha knockout mice. The number of tumors was determined weekly. Tumor types were determined by Hematoxylin and eosin (H & E) analysis. Tumor growth was assayed by proliferating cell nuclear antigen (PCNA) staining. RESULTS: In the knockout mice, the average number of tumors was 16.6/mouse at 20 weeks. In contrast, in the wild-type (WT) mice, the tumor number was 6.9/mouse. Growth and malignant grade of tumors in PKCalpha knockout mice did not differ from those in WT mice. CONCLUSION: PKCalpha suppresses tumor formation, but not tumor growth and progression in skin carcinogenesis. |
