| Primary Identifier | MGI:3603415 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Sgk3 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector was designed to insert a PGK-neo in place of exons 8 and 9, two critical exons for the kinase domain containing the catalytic core, including a lysine residue that is essential for ATP binding. Stop codons were inserted downstream of the exon 8 splice acceptor site. Northern blot showed a marked reduction in transcripts in mutant skin. Western blot did not detect protein in mutant mice. |
