| First Author | Lee DJ | Year | 2011 |
| Journal | Invest Ophthalmol Vis Sci | Volume | 52 |
| Issue | 12 | Pages | 8862-7 |
| PubMed ID | 21989727 | Mgi Jnum | J:189454 |
| Mgi Id | MGI:5445833 | Doi | 10.1167/iovs.11-8153 |
| Citation | Lee DJ, et al. (2011) Following EAU recovery there is an associated MC5r-dependent APC induction of regulatory immunity in the spleen. Invest Ophthalmol Vis Sci 52(12):8862-7 |
| abstractText | PURPOSE: IRBPp-specific regulatory immunity is found in the spleens of mice recovered from experimental autoimmune uveoretinitis (EAU). Induction of this regulatory immunity is dependent on the expression of the melanocortin 5 receptor (MC5r). Therefore, the authors investigated whether dependence on the expression of MC5r was with the T cells or with the APCs mediating protective regulatory immunity in the EAU-recovered mouse spleen. METHODS: Wild-type and MC5r-/- mice were immunized to induce EAU. The IRBPp-stimulated T-cell response in spleens of wild-type and MC5r-/- mice were compared for surface markers and cytokine production. Spleen APC were isolated and used to stimulate cytokine production and regulatory activity in IRBP-specific T cells from wild-type or MC5r-/- mice assayed in culture by ELISA, by flow cytometry, and in vivo by adoptive transfer into EAU mice. RESULTS: IRBPp-specific CD25+CD4+ T cells from spleens of EAU-recovered wild-type mice express a Treg cell phenotype of FoxP3 and TGF-beta compared with the effector T-cell phenotype of IFN-gamma and IL-17 production in EAU-recovered MC5r-/- mice. APCs from the spleens of wild-type mice recovering from EAU promoted regulatory T-cell activation in IRBP-specific effector T cells from the spleens of EAU-recovering MC5r-/- mice. Spleen APCs from EAU-recovering wild-type, but not MC5r-/-, mice induced TGF-beta expression by primed IRBP-specific effector T cells. CONCLUSIONS: Dependence on MC5r expression is with an APC that promotes or selectively activates IRBP-specific FoxP3+ TGF-beta+ CD25+CD4+ Treg cells in the spleens of EAU-recovered mice. |
