| First Author | Chakraborty R | Year | 2015 |
| Journal | Exp Eye Res | Volume | 137 |
| Pages | 79-83 | PubMed ID | 26072023 |
| Mgi Jnum | J:230778 | Mgi Id | MGI:5763735 |
| Doi | 10.1016/j.exer.2015.06.009 | Citation | Chakraborty R, et al. (2015) ON pathway mutations increase susceptibility to form-deprivation myopia. Exp Eye Res 137:79-83 |
| abstractText | The ON pathway mutation in nob mice is associated with altered refractive development, and an increased susceptibility to form-deprivation (FD) myopia. In this study, we used mGluR6-/- mice, another ON pathway mutant, to determine whether the nob phenotype was due to the Nyx mutation or abnormal ON pathway transmission. Refractive development under a normal visual environment for mGluR6-/- and age-matched wild-type (WT) mice was measured every 2 weeks from 4 to 16 weeks of age. The response to monocular FD from 4 weeks of age was measured weekly in a separate cohort of mice. Refraction and ocular biometry were obtained using a photorefractor and optical coherence tomography. Retinas were harvested at 16 weeks, and analyzed for dopamine (DA) and DOPAC using high-performance liquid chromatography. Under normal conditions, mGluR6-/- mice were significantly more myopic than their WT controls (refraction at 12 weeks; WT: 9.40 +/- 0.16 D, mGluR6-/-: 6.91 +/- 0.38 D). Similar to nob mice, two weeks of FD resulted in a significant myopic shift of -5.57 +/- 0.72 D in mGluR6-/- mice compared to -1.66 +/- 0.19 D in WT animals. No significant axial length changes were observed with either normal or FD visual conditions. At 16 weeks, mGluR6-/- retinas showed significantly lower DOPAC levels (111.2 +/- 33.0 pg/mg) compared to their WT counterparts (197.5 +/- 11.2 pg/mg). Retinal DA levels were similar between the different genotypes. Our results indicate that reduced retinal DA metabolism/turnover may be associated with increased susceptibility to myopia in mice with ON pathway defect mutations. |
