| Primary Identifier | MGI:3609482 | Allele Type | Targeted |
| Attribute String | Inserted expressed sequence, Reporter, Transactivator | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cell line = EB3, a mutation of ES cell line E14TG2a. EB3 cells carry one allele of Pou5f1 that has been inactivated by targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette. |
| molecularNote | EB3 ES cells were targeted with a vector containing the Tet-off regulation cassette for the homeodomain transcription factor Ipf1 and EGFP expression. A floxed neo was removed via cre-mediated excision. EB3 cells carry one allele of Pou5f1 that has been inactivated by targeted integration of E14Tg2a ES cells with an IRESBSDpA selection cassette. |
