| Primary Identifier | MGI:3609633 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Nr1i2 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A neomycin resistance gene replaced the first coding exon, which includes the first zinc finger of the DNA-binding domain, via homologous recombination. Northern blot and RT-PCR detected transcript ~200 bp shorter than the wild-type transcript, as expected. Sequence analysis revealed that exon 1 was fused to exon 3 in the mutant animal. The truncated transcript was present at reduced levels. If this RNA is translated from a spurious initiation site, the protein is expected to be nonfunctional. |
