| First Author | Weng PL | Year | 2016 |
| Journal | Sci Rep | Volume | 6 |
| Pages | 38199 | PubMed ID | 27910949 |
| Mgi Jnum | J:259982 | Mgi Id | MGI:6102250 |
| Doi | 10.1038/srep38199 | Citation | Weng PL, et al. (2016) Ascl3 transcription factor marks a distinct progenitor lineage for non-neuronal support cells in the olfactory epithelium. Sci Rep 6:38199 |
| abstractText | The olfactory epithelium (OE) is composed of olfactory sensory neurons (OSNs), sustentacular supporting cells, and several types of non-neuronal cells. Stem and progenitor cells are located basally, and are the source of all cell types needed to maintain OE homeostasis. Here, we report that Ascl3, a basic helix-loop-helix transcription factor, is expressed in the developing OE. Lineage tracing experiments demonstrate that the non-neuronal microvillar cells and Bowman''s glands are exclusively derived from Ascl3(+) progenitor cells in the OE during development. Following chemically-induced injury, Ascl3 expression is activated in a subset of horizontal basal cells (HBCs), which repopulate all microvillar cells and Bowman''s glands during OE regeneration. After ablation of Ascl3-expressing cells, the OE can regenerate, but lacks the non-neuronal microvillar and Bowman''s gland support cells. These results demonstrate that Ascl3 marks progenitors that are lineage-committed strictly to microvillar cells and Bowman''s glands, and highlight the requirement for these cell types to support OE homeostasis. |
