| Primary Identifier | MGI:3611771 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Car14 |
| Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<Sl-J>/J |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cell line = W9.5s |
| molecularNote | A targeting vector was used to replace exon 4, 16 bp of intron 3, and 13 bp of intron 4 with the self-excising tACE-CRE-neo cassette. The frameshift resulting from recombination putatively results in early termination in exon 5. RT-PCR failed to detect the predicted 0.75 fragment in brain, heart, kidney, liver or muscle samples. Protein was not detected in Western blot analysis of the same tissues. |
