| First Author | Sugimoto Y | Year | 2019 |
| Journal | Biochem Biophys Res Commun | Volume | 508 |
| Issue | 2 | Pages | 480-486 |
| PubMed ID | 30503339 | Mgi Jnum | J:291103 |
| Mgi Id | MGI:6442689 | Doi | 10.1016/j.bbrc.2018.11.089 |
| Citation | Sugimoto Y, et al. (2019) Prediction of intracellular targets of a small compound by analyzing peptides presented on MHC class I. Biochem Biophys Res Commun 508(2):480-486 |
| abstractText | In chemical biology, the elucidation of chemical target is crucial for successful drug development. Because MHC class I molecules present peptides from intracellular damaged proteins, it might be possible to identify targets of a chemical by analyzing peptide sequences on MHC class I. Therefore, we treated cells with the autophagy-inducing chemical TMD-457 and identified the peptides presented on MHC class I. Many of the peptides were derived from molecules involved in ER trafficking and ER stress, which were confirmed by morphological and biochemical analyses. Therefore, our results demonstrate that analyzing MHC class I peptides is useful for the detection of chemical targets. |
