| Primary Identifier | MGI:3615270 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Tcrg |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting construct was designed to replace the T-cell receptor gamma variable 1 gene as well as the joining region and exons 1 and 2 of the T-cell receptor gamma constant 4 gene with a neomycin resistance cassette. Removal of this region would prevent any rearrangement and the possibility of pairing other variable gamma genes with an otherwise intact constant 4 gene. Absence of Tcrg-V1 mRNA in RT-PCR of mutant spleen and thymus confirmed successful ablation. |
