| Primary Identifier | MGI:3619022 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Cacna1e |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A floxed neomycin cassette was inserted between exons 2 and 3 and a third loxP site was inserted upstream of exon 2. The neomycin cassette was subsequently removed from properly targeted ES cells by transient transfection with a cre expressing vector. As a result, exon 2 was flanked by loxP sites. The resulting mice were crossed with mice carrying Tg(CMV-cre)1Cgn which caused the germline excision of exon 2. |
