| Primary Identifier | MGI:3622072 | Allele Type | Targeted |
| Gene | Igh-8 | Transmission | Germline |
| Strain of Origin | 129S2/SvPas | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | A targeting vector was designed to insert a neomycin resistance gene in a sense orientation downstream of the Igamma3 exon, leaving intact all the necessary elements for proper transcription of the gene. Mutants demonstrated a significant impairment In IgG3, but not IgM or IgG2b production. Transcripts of the Igamma3-neo were not detected in RT-PCR, suggesting that the germ line transcription from the promoter was impaired. However, transcript of neo-Cgamma3 was detected, resulting from the TK promoter, and demonstrating that two novel cryptic splice sites were activated, one in the neo and the other in the Igamma3-Cgamma3 intron. |
