| Primary Identifier | MGI:3623144 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Psme4 |
| Transmission | Germline | Strain of Origin | 129 x C57BL/6 |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cell line = F1BS |
| molecularNote | A floxed neo was inserted immediately upstream of exon 2 using one targeting vector. The ES cells were targeted again to insert a floxed neo immediately downstream of exon 48 of exon 2 using one targeting vector. Cre-recombinase interchromosomal recombination resulted in the loss of most of the locus, leaving only the first coding exon. At most, the mutant locus would encode the N-terminal 80 amino acids of the protein. Northern blot did not detect mRNA from mutants. Western blot confirmed absence of protein. |
