| First Author | Kummola L | Year | 2017 |
| Journal | J Immunol | Volume | 198 |
| Issue | 10 | Pages | 3909-3918 |
| PubMed ID | 28404633 | Mgi Jnum | J:247869 |
| Mgi Id | MGI:5926918 | Doi | 10.4049/jimmunol.1600753 |
| Citation | Kummola L, et al. (2017) IL-7Ralpha Expression Regulates Murine Dendritic Cell Sensitivity to Thymic Stromal Lymphopoietin. J Immunol 198(10):3909-3918 |
| abstractText | Thymic stromal lymphopoietin (TSLP) and IL-7 are related cytokines that mediate growth and differentiation events in the immune system. They signal through IL-7Ralpha-containing receptors. Target cells of TSLP in Th2 responses include CD4 T cells and dendritic cells (DCs). Although it has been reported that expression of TSLP receptor (TSLPR) on CD4 T cells is required for OVA-induced lung inflammation, DCs have also been shown to be target cells of TSLP. In this study, we show that murine ex vivo splenic DCs are unresponsive to TSLP, as they fail to phosphorylate STAT5, but in vitro overnight culture, especially in presence of IL-4, renders DCs responsive to both TSLP and IL-7. This induced responsiveness is accompanied by dramatic upregulation of IL-7Ralpha on DCs with little change in expression of TSLPR or of gammac In splenic DCs, the induction of IL-7Ralpha occurs mainly in CD8- DCs. In vivo, we found that IL-4 has a differential regulatory role on expression of IL-7Ralpha depending on the cell type; IL-4 decreases IL-7Ralpha expression on CD4 T cells whereas it upregulates the expression on DCs. Our results indicate that the induction of IL-7Ralpha expression on DCs is critical for TSLP responsiveness and that IL-4 can upregulate IL-7Ralpha on DCs. |
