| Primary Identifier | MGI:3625158 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Cadm1 |
| Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | ES cells targeted with a vector designed to flank exon 9 with loxP sites and insert an FRT-flanked neo were transfected with Cre recombinase. The floxed region including exon 9 and the neo were removed. Exon 9 encodes the transmembrane domain. The skipping of exon 9 resulted in a reading frame shift, which was predicted to result in a nonsense transcript. RT-PCR confirmed that the mutant allele was null. Immunohistochemistry demonstrated the absence of protein in mutant testis. |
