| First Author | Banerjee A | Year | 2016 |
| Journal | PLoS One | Volume | 11 |
| Issue | 5 | Pages | e0155311 |
| PubMed ID | 27167731 | Mgi Jnum | J:248946 |
| Mgi Id | MGI:6094402 | Doi | 10.1371/journal.pone.0155311 |
| Citation | Banerjee A, et al. (2016) YY1 Is Required for Germinal Center B Cell Development. PLoS One 11(5):e0155311 |
| abstractText | YY1 has been implicated as a master regulator of germinal center B cell development as YY1 binding sites are frequently present in promoters of germinal center-expressed genes. YY1 is known to be important for other stages of B cell development including the pro-B and pre-B cells stages. To determine if YY1 plays a critical role in germinal center development, we evaluated YY1 expression during B cell development, and used a YY1 conditional knock-out approach for deletion of YY1 in germinal center B cells (CRE driven by the immunoglobulin heavy chain gamma1 switch region promoter; gamma1-CRE). We found that YY1 is most highly expressed in germinal center B cells and is increased 3 fold in splenic B cells activated by treatment with anti-IgM and anti-CD40. In addition, deletion of the yy1 gene by action of gamma1-CRE recombinase resulted in significant loss of GC cells in both un-immunized and immunized contexts with corresponding loss of serum IgG1. Our results show a crucial role for YY1 in the germinal center reaction. |
