| First Author | Suzuki H | Year | 2013 |
| Journal | Genesis | Volume | 51 |
| Issue | 1 | Pages | 50-8 |
| PubMed ID | 22965810 | Mgi Jnum | J:191888 |
| Mgi Id | MGI:5463526 | Doi | 10.1002/dvg.22347 |
| Citation | Suzuki H, et al. (2013) Generation of a germ cell-specific mouse transgenic cherry reporter, Sohlh1-mCherryFlag. Genesis 51(1):50-8 |
| abstractText | Visualization of differentiating germ cells is critical to understanding the formation of primordial follicles in the ovary, and the commitment of spermatogonial stem cells to differentiation. We engineered and generated a BAC transgenic mouse line, Sohlh1-mCherryFlag (S1CF), under the direction of the native Sohlh1 promoter. Sohlh1 is a germ cell-specific gene that encodes the basic helix-loop-helix (bHLH) transcriptional regulator that is essential in oogenesis and spermatogenesis. Sohlh1 expression is unique, and is limited to perinatal and early follicle oocytes and differentiating spermatogonia. The Sohlh1-mCherryFlag transgene was engineered to fuse SOHLH1 to the red fluorescent protein CHERRY with 3-tandem-FLAG tags. S1CF animals fluoresce specifically in the oocytes of perinatal ovaries and small follicles in adult ovaries, as well as in spermatogonia, a pattern that is similar to endogenous SOHLH1. Moreover, S1CF rescued germ cell loss and infertility in both male and female Sohlh1(-/-) animals. The FLAG-tag on S1CF was effective for immunostaining and immunoprecipitation. The Sohlh1-mCherryFlag transgenic mouse provides a unique model to study early germ cell differentiation, as well as in vivo imaging and purification of differentiating germ cells. genesis 51:50-58, 2013. (c) 2012 Wiley Periodicals, Inc. |
