| First Author | Lipp S | Year | 2009 |
| Journal | Neurosci Lett | Volume | 457 |
| Issue | 2 | Pages | 85-8 |
| PubMed ID | 19429168 | Mgi Jnum | J:150441 |
| Mgi Id | MGI:3850779 | Doi | 10.1016/j.neulet.2009.04.001 |
| Citation | Lipp S, et al. (2009) Calcium responses mediated by type 2 IP3-receptors are required for osmotic volume regulation of retinal glial cells in mice. Neurosci Lett 457(2):85-8 |
| abstractText | Prevention of osmotic swelling of retinal glial (Muller) cells is required to avoid detrimental decreases in the extracellular space volume during intense neuronal activity. Here, we show that glial cells in slices of the wildtype mouse retina maintain the volume of their somata constant up to approximately 4 min of perfusion with a hypoosmolar solution. However, calcium chelation with BAPTA/AM induced a rapid swelling of glial cell bodies. In glial cells of retinas from inositol-1,4,5-trisphosphate-receptor type 2-deficient (IP(3)R2(-/-)) mice, hypotonic conditions caused swelling of the cell bodies without delay. Exogenous ATP (acting at P2Y(1) receptors) prevented the swelling of glial cells in retinal slices from wildtype but not from IP(3)R2(-/-) mice. Muller cells from IP(3)R2(-/-) mice displayed a strongly reduced amplitude of the ATP-evoked calcium responses as compared to cells from wildtype mice. It is concluded that endogenous calcium signaling mediated by IP(3)R2 is required for the osmotic volume regulation of retinal glial cells. |
