| First Author | Klein B | Year | 2020 |
| Journal | Sci Rep | Volume | 10 |
| Issue | 1 | Pages | 20095 |
| PubMed ID | 33208869 | Mgi Jnum | J:300237 |
| Mgi Id | MGI:6491100 | Doi | 10.1038/s41598-020-77115-w |
| Citation | Klein B, et al. (2020) DCX(+) neuronal progenitors contribute to new oligodendrocytes during remyelination in the hippocampus. Sci Rep 10(1):20095 |
| abstractText | A pool of different types of neural progenitor cells resides in the adult hippocampus. Apart from doublecortin-expressing (DCX(+)) neuronal progenitor cells (NPCs), the hippocampal parenchyma also contains oligodendrocyte precursor cells (OPCs), which can differentiate into myelinating oligodendrocytes. It is not clear yet to what extent the functions of these different progenitor cell types overlap and how plastic these cells are in response to pathological processes. The aim of this study was to investigate whether hippocampal DCX(+) NPCs can generate new oligodendrocytes under conditions in which myelin repair is required. For this, the cell fate of DCX-expressing NPCs was analyzed during cuprizone-induced demyelination and subsequent remyelination in two regions of the hippocampal dentate gyrus of DCX-CreER(T2)/Flox-EGFP transgenic mice. In this DCX reporter model, the number of GFP(+) NPCs co-expressing Olig2 and CC1, a combination of markers typically found in mature oligodendrocytes, was significantly increased in the hippocampal DG during remyelination. In contrast, the numbers of GFP(+)PDGFRalpha(+) cells, as well as their proliferation, were unaffected by de- or remyelination. During remyelination, a higher portion of newly generated BrdU-labeled cells were GFP(+) NPCs and there was an increase in new oligodendrocytes derived from these proliferating cells (GFP(+)Olig2(+)BrdU(+)). These results suggest that DCX-expressing NPCs were able to contribute to the generation of mature oligodendrocytes during remyelination in the adult hippocampus. |
