| Primary Identifier | MGI:3653481 | Allele Type | Targeted |
| Attribute String | Modified regulatory region, Null/knockout | Gene | Rr165 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector was designed to mutate the KI-KII element (Rr163) such that 8 and 9 bp were replaced, respectively. The sequence of KI was mutated from TCCACGCATGCTTGGA to TGTACGCTGCTGATGA and KII was mutated from TCCTCTTCAGTGAGGAG to AGCTCTTCTAGACTGA. LoxP sites were inserted to surround 4 kb encompassing the promoter of the long J-C K germline transcript, located immediately upstream of the KI-KII element. This sequence was deleted through subsequent cre-mediated recombination. |
