| Primary Identifier | MGI:3653518 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Als2 |
| Transmission | Germline | Strain of Origin | 129S1/SvImJ |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A promoter trap strategy was used to replace exons 3 and 4 with an SA-IRES-betageo-pA cassette. The resulting protein encoded from the locus would contain only the five N-terminal amino acids fused to lacZ-neo, thereby inactivating both the full-length and the proposed short form of amyotrophic lateral sclerosis 2 (juvenile) homolog. Absence of protein was confirmed by Western blot analysis of cortex, cerebellum and testis. |
