| Primary Identifier | MGI:3656025 | Allele Type | Transgenic |
| Attribute String | Inducible, Inserted expressed sequence, Reporter | Gene | Tg(tetO-Aurkb,lacZ)41Kra |
| Strain of Origin | FVB/NJ | Induced With | doxycycline/tetracycline |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A vector pBI-G (Clontech #6150-1) was used to create this transgene. The vector contains a prokaryotic beta-galactosidase (lacZ) reporter gene under the control of the tetracycline-response element (TRE; containing seven direct repeats of the tetracycline operator (tetO) sequence fused to bidirectional cytomegalovirus (CMV) minimal promoter). The rat Aurora-kinase B cDNA (with N-terminal FLAG-tag sequence and 3' linked beta-globin gene) was placed downstream of the promoter and the purified construct was injected into fertilized FVB/NJ eggs to create the transgenic mice. |
