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Allele : Tg(CAG-mRFP1)1F1Hadj transgene insertion 1F1, Anna-Katerina Hadjantonakis
Primary Identifier  MGI:3663356 Allele Type  Transgenic
Attribute String  Reporter Gene  Tg(CAG-mRFP1)1F1Hadj
Transmission  Germline Strain of Origin  (129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Is Recombinase  false Is Wild Type  false
molecularNote  The first true monomeric red fluorescent protein (mRFP1) is the result of the stepwise directed evolution of DsRed; introducing 33 amino acid substitutions selecting for improved performance and developmental neutrality. The mRFP1 coding sequence was placed under control of the chicken beta actin promoter and cytomegalovirus (CMV) immediate early enhancer combination, designed to drive high-level constitutive gene expression in ES cells, embryos and adult mice. The construct (CAG-mRFP1) was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Mice hemizygous for the transgene exhibit robust and widespread expression of monomeric red fluorescent protein-1 (mRFP1) in embryonic stem cells, embryos, and adults.
  • mutations:
  • Insertion
  • synonyms:
  • Tg(ACTB-mRFP1)1F1Hadj,
  • Tg(ACTB-mRFP1)1F1Hadj,
  • CAG::mRFP1,
  • CAG::mRFP1
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Trail: Allele

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0 Mutation Involves

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Trail: Allele




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