| First Author | Leeman KT | Year | 2019 |
| Journal | Sci Rep | Volume | 9 |
| Issue | 1 | Pages | 6479 |
| PubMed ID | 31015509 | Mgi Jnum | J:278633 |
| Mgi Id | MGI:6357598 | Doi | 10.1038/s41598-019-42819-1 |
| Citation | Leeman KT, et al. (2019) Mesenchymal Stem Cells Increase Alveolar Differentiation in Lung Progenitor Organoid Cultures. Sci Rep 9(1):6479 |
| abstractText | Lung epithelial cell damage and dysfunctional repair play a role in the development of lung disease. Effective repair likely requires the normal functioning of alveolar stem/progenitor cells. For example, we have shown in a mouse model of bronchopulmonary dysplasia (BPD) that mesenchymal stem cells (MSC) protect against hyperoxic lung injury at least in part by increasing the number of Epcam(+) Sca-1(+) distal lung epithelial cells. These cells are capable of differentiating into both small airway (CCSP(+)) and alveolar (SPC(+)) epithelial cells in three-dimensional (3D) organoid cultures. To further understand the interactions between MSC and distal lung epithelial cells, we added MSC to lung progenitor 3D cultures. MSC stimulated Epcam(+) Sca-1(+) derived organoid formation, increased alveolar differentiation and decreased self-renewal. MSC-conditioned media was sufficient to promote alveolar organoid formation, demonstrating that soluble factors secreted by MSC are likely responsible for the response. This work provides strong evidence of a direct effect of MSC-secreted factors on lung progenitor cell differentiation. |
