| First Author | Monteiro JT | Year | 2019 |
| Journal | Viruses | Volume | 11 |
| Issue | 3 | PubMed ID | 30917612 |
| Mgi Jnum | J:291134 | Mgi Id | MGI:6442942 |
| Doi | 10.3390/v11030303 | Citation | Monteiro JT, et al. (2019) The CARD9-Associated C-Type Lectin, Mincle, Recognizes La Crosse Virus (LACV) but Plays a Limited Role in Early Antiviral Responses against LACV. Viruses 11(3) |
| abstractText | La Crosse virus (LACV) is a mosquito-transmitted arbovirus and the main cause of virus-mediated neurological diseases in children. To date, little is known about the role of C-type lectin receptors (CLRs)-an important class of pattern recognition receptors-in LACV recognition. DC-SIGN remains the only well-described CLR that recognizes LACV. In this study, we investigated the role of additional CLR/LACV interactions. To this end, we applied a flow-through chromatography method for the purification of LACV to perform an unbiased high-throughput screening of LACV with a CLR-hFc fusion protein library. Interestingly, the CARD9-associated CLRs Mincle, Dectin-1, and Dectin-2 were identified to strongly interact with LACV. Since CARD9 is a common adaptor protein for signaling via Mincle, Dectin-1, and Dectin-2, we performed LACV infection of Mincle(-/-) and CARD9(-/-) DCs. Mincle(-/-) and CARD9(-/-) DCs produced less amounts of proinflammatory cytokines, namely IL-6 and TNF-alpha, albeit no reduction of the LACV titer was observed. Together, novel CLR/LACV interactions were identified; however, the Mincle/CARD9 axis plays a limited role in early antiviral responses against LACV. |
