| First Author | Nikolova G | Year | 2008 |
| Journal | Genetics | Volume | 179 |
| Issue | 1 | Pages | 419-27 |
| PubMed ID | 18458101 | Mgi Jnum | J:135195 |
| Mgi Id | MGI:3790578 | Doi | 10.1534/genetics.108.088088 |
| Citation | Nikolova G, et al. (2008) The Chromosome 11 Region From Strain 129 Provides Protection From Sex Reversal in XYPOS Mice. Genetics 179(1):419-27 |
| abstractText | C57BL/6J (B6) mice containing the Mus domesticus poschiavinus Y chromosome, Y(POS), develop ovarian tissue, whereas testicular tissue develops in DBA/2J or 129S1/SvImJ (129) mice containing the Y(POS) chromosome. To identify genes involved in sex determination, we used a congenic strain approach to determine which chromosomal regions from 129Sl/SvImJ provide protection against sex reversal in XY(POS) mice of the C57BL/6J.129-Y(POS) strain. Genome scans using microsatellite and SNP markers identified a chromosome 11 region of 129 origin in C57BL/6J.129-Y(POS) mice. To determine if this region influenced testis development in XY(POS) mice, two strains of C57BL/6J-Y(POS) mice were produced and used in genetic experiments. XY(POS) adults homozygous for the 129 region had a lower incidence of sex reversal than XY(POS) adults homozygous for the B6 region. In addition, many homozygous 129 XY(POS) fetuses developed normal-appearing testes, an occurrence never observed in XY(POS) mice of the C57BL/6J-Y(POS) strain. Finally, the amount of testicular tissue observed in ovotestes of heterozygous 129/B6 XY(POS) fetuses was greater than the amount observed in ovotestes of homozygous B6 XY(POS) fetuses. We conclude that a chromosome 11 locus derived from 129Sl/SvImJ essentially protects against sex reversal in XY(POS) mice. A number of genes located in this chromosome 11 region are discussed as potential candidates. |
