| First Author | Kashani E | Year | 2015 |
| Journal | Nat Commun | Volume | 6 |
| Pages | 6477 | PubMed ID | 25765849 |
| Mgi Jnum | J:221884 | Mgi Id | MGI:5641796 |
| Doi | 10.1038/ncomms7477 | Citation | Kashani E, et al. (2015) A clonotypic Vgamma4Jgamma1/Vdelta5Ddelta2Jdelta1 innate gammadelta T-cell population restricted to the CCR6(+)CD27(-) subset. Nat Commun 6:6477 |
| abstractText | Here we investigate the TCR repertoire of mouse Vgamma4(+) gammadelta T cells in correlation with their developmental origin and homeostasis. By deep sequencing we identify a high frequency of straight Vdelta5Ddelta2Jdelta1 germline rearrangements without P- and N-nucleotides within the otherwise highly diverse Trd repertoire of Vgamma4(+) cells. This sequence is infrequent in CCR6(-)CD27(+) cells, but abundant among CCR6(+)CD27(-) gammadelta T cells. Using an inducible Rag1 knock-in mouse model, we show that gammadelta T cells generated in the adult thymus rarely contain this germline-rearranged Vdelta5Ddelta2Jdelta1 sequence, confirming its fetal origin. Single-cell analysis and deep sequencing of the Trg locus reveal a dominant CDR3 junctional motif that completes the TCR repertoire of invariant Vgamma4(+)Vdelta5(+) cells. In conclusion, this study identifies an innate subset of fetal thymus-derived gammadelta T cells with an invariant Vgamma4(+)Vdelta5(+) TCR that is restricted to the CCR6(+)CD27(-) subset of gammadelta T cells. |
