| First Author | Cho KJ | Year | 2020 |
| Journal | J Immunol | Volume | 204 |
| Issue | 6 | Pages | 1621-1629 |
| PubMed ID | 31996461 | Mgi Jnum | J:287318 |
| Mgi Id | MGI:6405804 | Doi | 10.4049/jimmunol.1901234 |
| Citation | Cho KJ, et al. (2020) Activation of Dendritic Cells Alters the Mechanism of MHC Class II Antigen Presentation to CD4 T Cells. J Immunol 204(6):1621-1629 |
| abstractText | Both immature and mature dendritic cells (DCs) can process and present foreign Ags to CD4 T cells; however, the mechanism by which MHC class II (MHC-II) in mature DCs acquires antigenic peptides remains unknown. To address this, we have studied Ag processing and presentation of two distinct CD4 T cell epitopes of the influenza virus hemagglutinin coat protein by both immature and mature mouse DCs. We find that immature DCs almost exclusively use newly synthesized MHC-II targeted to DM(+) late endosomes for presentation to influenza virus-specific CD4 T cells. By contrast, mature DCs exclusively use recycling MHC-II that traffics to both early and late endosomes for antigenic peptide binding. Rab11a knockdown partially inhibits recycling of MHC-II in mature DCs and selectively inhibits presentation of an influenza virus hemagglutinin CD4 T cell epitope generated in early endosomes. These studies highlight a "division of labor" in MHC-II peptide binding, in which immature DCs preferentially present Ags acquired in Rab11a(-) DM(+) late endosomes, whereas mature DCs use recycling MHC-II to present antigenic peptides acquired in both Rab11a(+) early endosomes and Rab11a(-) endosomes for CD4 T cell activation. |
