| First Author | Krishnamoorthy M | Year | 2018 |
| Journal | Sci Signal | Volume | 11 |
| Issue | 533 | PubMed ID | 29871911 |
| Mgi Jnum | J:280850 | Mgi Id | MGI:6376423 |
| Doi | 10.1126/scisignal.aan2693 | Citation | Krishnamoorthy M, et al. (2018) The ion channel TRPM7 is required for B cell lymphopoiesis. Sci Signal 11(533) |
| abstractText | The transient receptor potential (TRP) family is a large family of widely expressed ion channels that regulate the intracellular concentration of ions and metals and respond to various chemical and physical stimuli. TRP subfamily M member 7 (TRPM7) is unusual in that it contains both an ion channel and a kinase domain. TRPM7 is a divalent cation channel with preference for Ca(2+) and Mg(2+) It is required for the survival of DT40 cells, a B cell line; however, deletion of TRPM7 in T cells does not impair their development. We found that expression of TRPM7 was required for B cell development in mice. Mice that lacked TRPM7 in B cells failed to generate peripheral B cells because of a developmental block at the pro-B cell stage. The loss of TRPM7 kinase activity alone did not affect the proportion of peripheral mature B cells or the development of B cells in the bone marrow. However, supplementation with a high concentration of extracellular Mg(2+) partially rescued the development of TRPM7-deficient B cells in vitro. Thus, our findings identify a critical role for TRPM7 ion channel activity in B cell development. |
