| First Author | Diaz-Muñoz MD | Year | 2015 |
| Journal | Nat Immunol | Volume | 16 |
| Issue | 4 | Pages | 415-25 |
| PubMed ID | 25706746 | Mgi Jnum | J:231498 |
| Mgi Id | MGI:5771639 | Doi | 10.1038/ni.3115 |
| Citation | Diaz-Munoz MD, et al. (2015) The RNA-binding protein HuR is essential for the B cell antibody response. Nat Immunol 16(4):415-25 |
| abstractText | Post-transcriptional regulation of mRNA by the RNA-binding protein HuR (encoded by Elavl1) is required in B cells for the germinal center reaction and for the production of class-switched antibodies in response to thymus-independent antigens. Transcriptome-wide examination of RNA isoforms and their abundance and translation in HuR-deficient B cells, together with direct measurements of HuR-RNA interactions, revealed that HuR-dependent splicing of mRNA affected hundreds of transcripts, including that encoding dihydrolipoamide S-succinyltransferase (Dlst), a subunit of the 2-oxoglutarate dehydrogenase (alpha-KGDH) complex. In the absence of HuR, defective mitochondrial metabolism resulted in large amounts of reactive oxygen species and B cell death. Our study shows how post-transcriptional processes control the balance of energy metabolism required for the proliferation and differentiation of B cells. |
