| Primary Identifier | MGI:3690080 | Allele Type | Targeted |
| Attribute String | Dominant negative, Reporter | Gene | Pax3 |
| Transmission | Germline | Strain of Origin | 129S2/SvPas |
| Is Recombinase | false | Is Wild Type | false |
| description | This allele was generated in J:112275 using a cross between females carrying Tg(Pgk1-cre)1Lni and males carrying the floxed allele. As this cre transgene is expressed in diploid eggs, all offspring are exposed to Cre protien, independent of inheritance of the cre transgene. |
| molecularNote | Cre mediated recombination removed the floxed GFP-FRT-puro cassette from Pax3tm5Buck allowing expresion of a form of Pax3 that acts as a transcriptional repressor. The repressor sequence contains the N-terminus of the Pax3 DNA-binding domains fused to the repression domain of the Drosophila Engrailed transcriptional repressor. LacZ expression is seen in Pax3 expressing cells. |
